The principles of the 3Rs established by Russel and Burch in 1959 aim to replace, reduce and refine the use of animals in research worldwide. Since then, it has become important to think of methodologies that can bring the same results that tests on live animals bring, but obviously without using them. Therefore, alternative methods to the use of animals is related to the development and implementation of methodologies that perform tests without using animals.

The National Council for the Control of Animal Experimentation (CONCEA) established, in Normative Resolution 17, as an alternative method like any method that can be used to replace, reduce or refine the use of animals in research activities. These alternative methods are considered validated when the method has reliability and relevance through a process that involves the stages of development, pre-validation, validation and review by specialists. Thus, the validated methods are normally performed by the Alternative Methods Validation Center or by international collaborative studies, and may have international regulatory acceptance. In Brazil, the Brazilian Center for Validation of Alternative Methods (BraCVAM) is responsible for validating alternative methods to the use of animals for experimentation and education.

Finally, alternative methods recognized as validated alternative methods that have been recognized by CONCEA are considered. Currently, CONCEA has already recognized 24 alternative methodologies. In addition to these, there is a continuous effort to develop new alternative methodologies in the areas of cell culture in vitro and computational models in silico so that we increasingly reduce the use of experimental animals.